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Strain specificity of yeasts isolated from different locations of women suffering from vaginal candidosis, and their partners
Vergleich der Stammspezifität von Hefepilzen verschiedener Lokalisation bei Frauen mit Vaginalcandidosen
Author(s) -
Mendling W.,
Pinto De Andrade M.,
Gutschmidt J.,
Gantenberg R.,
Presber W.,
Schönian Gabriele
Publication year - 2000
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2000.00604.x
Subject(s) - vagina , microbiology and biotechnology , biology , candida albicans , agar , feces , semen , corpus albicans , yeast , bacteria , anatomy , genetics
Summary. Sexual partners often harbour identical yeast strains in the vagina, in the orointestinal tract and in semen in cases of recurrent vulvovaginal candidoses. Specimen were collected from vagina, oral cavity and faeces of the patients, and from semen, oral cavity and faeces of their male partners. Mycological cultures were grown on Sabouraud glucose‐agar and, if positive, specified by Candida ‐ID‐Agar (BioMérieux), by formation of chlamydospores on rice agar, and by biochemotyping with the System Walk‐away (Dade) or the API‐32C system (BioMérieux). A polymerase chain reaction fingerprinting technique with the T3B oligonucleotide as single primer was used for strain typing. Candida albicans was isolated from the vagina of 18 out of 21 patients, the vagina of one patient harboured a strain of Candida glabrata . The cultures obtained from vagina, oral cavity and faeces were genetically identical in 12 patients. From the partners of 15 patients C. albicans was cultured in at least one of the clinical samples. Identical strains were observed for eight of 15 couples, whereas four of these identical strains were cultured from semen. Further prospective investigations will prove whether a consequent treatment of both partners will eradicate identical yeast strains and will be able to improve the results of treatment in such women.