Premium
Caco‐2‐Monolayer als Modell für die transepitheliale Migration von Candida albicans
Author(s) -
Weide M. R.,
Ernst J. F.
Publication year - 1999
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.1999.tb00015.x
Subject(s) - candida albicans , hypha , microbiology and biotechnology , mutant , ura3 , monolayer , biology , intracellular , chemistry , biochemistry , gene
Summary A model for transepithelial migration of human fungal pathogens was established, in which Candida albicans was shown to migrate across a monolayer of Caco‐2 intestinal cells in a two‐chamber system. Electron microscopy revealed typical stages of epithelial penetration by C. albicans including phagocytosis at the apical side, intra‐ and intercellular migration and exit on the basolateral side of the monolayer. Hyphal growth forms appeared particularly involved in penetration of the Caco‐2 monolayer. The model was examined using defined C. albicans mutants defective in hyphal development ( efg1/efg1 ) or growth ( ura3/ura3 ). Transmigration of the efg1/efg1 mutant strain was reduced, while transmigration of the ura3/ura3 strain was blocked completely in the absence of uridine. Because these results parallel virulence characteristics of the mutants the Caco‐2 monolayer system appears a useful model for the study of fungal‐human host cell interactions.