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The effect of sulconazole on the antigenic composition of two dermatophyte species
Author(s) -
Hearn Veronica M.,
Moore Mary K.,
Latgé J. P.,
Fracchia J. N.
Publication year - 1992
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.1992.tb00834.x
Subject(s) - dermatophyte , composition (language) , antigen , trichophyton , microbiology and biotechnology , biology , chemistry , immunology , antifungal , art , literature
Summary.Trichophyton rubrum and Trichophyton interdigitale have been grown in liquid culture in the presence of sulconazole. The antigenic activity of detergent extracts of intact organisms was analysed following SDS‐PAGE and the probing of Western blots with homologous antisera raised in rabbits and with sera from patients with dermatophyte infections. Differences in protein‐band patterns were noted; some bands present in control samples were absent in azole‐treated samples and vice versa. These differences were reflected in antigenic band patterns, especially among components of approximate molecular weight of 30–40, 50–60 and 92–100 kDa. Zusammenfassung.Trichophyton rubrum and Trichophyton interdigitale wurden in Gegenwart von Sulconazol in Flüssigkulturen angezüchtet. Der Antigenbestand in Detergens‐Extrakten der intakten Organismen wurde nach SDS‐PAGE in Westernblots unter Verwendung homologer Seren von Kaninchen und von Dermatophytose‐Patienten untersucht. Es wurden Unterschiede in den Proteinbanden gefunden. Einige Banden zeigten sich in den Kontrollproben, fehlten aber in den Sulconazol‐behandelten Ansätzen und umgekehrt. Diese Differenzen zeigten sich in Antigenbanden mit Molekulargewichten von 30‐40, 50‐60 und 92‐100 kDa.