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Zur enzymaktivitätshemmenden Wirkung der antimyzetisch wirksamen Imidazol‐Derivate
Author(s) -
Raab W.,
Högl F.
Publication year - 1981
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.1981.tb01903.x
Subject(s) - chemistry , alkaline phosphatase , phosphatase , enzyme , dehydrogenase , acid phosphatase , biochemistry , microbiology and biotechnology , econazole , phosphate , biology , miconazole , antifungal
Zusammenfassung: Zur weiteren Aufklärung des biochemischen Wirkmechanismus der Imidazol‐Derivate gegen Myzeten wurde das Verhalten einiger Enzymaktivitäten in Anwesenheit von Econazolnitrat untersucht: Die Glukose‐6‐phosphat‐Dehydrogenase, die “alkalische” Phosphatas, die “saure” Phosphatase, die Leucin‐Arylamidase und die β‐Glukuronidase. In gepoolten Humansera erfolgten keine Veränderungen der Enzymaktivitäten. Auch das Reinenzym “alkalische” Phosphatase blieb unbeeinflußt; hingegen fand sich an den Reinenzymen Glukose‐6‐Phosphat‐Dehydrogenase und “saure” Phosphatase ein dosisabhängiger Hemmeffekt des Econazolnitrats. Diese Hemmwirkung zeigte sich jedoch erst in Konzentrationen von oder über 10μg/ml. Summary: The various biochemical mechanisms by which antifungal imidazole derivatives develop their typical effects are still under discussion. As a contribution to this problem, the behaviour of enzymatic activities under the influence of econazole nitrate was investigated. The following enzymes were tested: glucose‐6‐phosphate dehydrogenase, “alkaline” phosphatase, “acid” phosphatase, leucine arylamidase, and β‐glucuronidase. In pooled human sera, econazole nitrate in concentrations up to 50 μg/ml remained without effect on enzymatic activities. Equally, the pure enzyme “alkaline” phosphatase was not inhibited. On pure glucose‐6‐phosphate dehydrogenase as well as on pure “acid” phosphatase, a significant dose‐dependant inhibition by econazole nitrate was found. This inhibitory action developed in concentrations of or above 10 μg/ml.