Open AccessCanine‐coronavirus (CCV) Characterization in Spain Epidemiological Aspects
Author(s) -
MartinCalvo Mar,
Marcotegui Miguel Angel,
Simarro Isabel
Publication year - 1994
Publication title -
journal of veterinary medicine, series b
Language(s) - English
Resource type - Journals
eISSN - 1439-0450
pISSN - 0931-1793
DOI - 10.1111/j.1439-0450.1994.tb00225.x
Subject(s) - virology , strain (injury) , coronavirus , antibody , biology , covid-19 , microbiology and biotechnology , medicine , pathology , immunology , disease , infectious disease (medical specialty) , anatomy
Summary In this paper the characterization of a canine‐coronavirus (CCV) strain isolated in Spain is reported. The CCV cellular‐infection cycle on A‐72 cells was studied using electron‐microscopy techniques. The isolate was found to have similar electrophoretic profile and antigenic properties to an American reference strain. Sera samples were obtained from several canine populations and tested for viral antibodies using two immunoenzymatic methods. Sera specificity was confirmed using immunoblot analysis. The agreement between both ELISA tests produced adequate results (kappa value = 0.64).