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Genetic S tructure of C ochliobolus sativus P opulations S ampled from R oots and L eaves of B arley and W heat in N orth D akota
Author(s) -
Gyawali Sanjaya,
Neate Stephen M.,
Adhikari Tika B.,
Puri Krishna D.,
Burlakoti Rishi R.,
Zhong Shaobin
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2012.01956.x
Subject(s) - cochliobolus sativus , biology , hordeum vulgare , amplified fragment length polymorphism , population , botany , genetic variation , linkage disequilibrium , genetic diversity , hordeum , horticulture , genetics , gene , cultivar , poaceae , genotype , single nucleotide polymorphism , demography , sociology
Abstract Common root rot ( CRR ) and spot blotch, caused by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dast., are important diseases of barley ( Hordeum vulgare L.) and wheat ( Triticum aestivum L.) worldwide. However, the population biology of C. sativus is still poorly understood. In this study, the genetic structure of three C. sativus populations, consisting of isolates sampled respectively from barley leaves ( BL ), barley roots ( BR ) and wheat roots ( WR ) in North Dakota, was analysed with amplified fragment length polymorphism ( AFLP ) markers. A total of 127 AFLP loci were generated among 208 C. sativus isolates analysed with three primer combinations. Gene diversity ( H = 0.277–0.335) were high in all three populations. Genetic variation among C. sativus individuals within population accounted for 74%, whereas 26% of the genetic variation was explained among populations. Genetic differentiation was high ( ØPT = 0.261, correctedG ' st'= 0.39), whereas gene flow ( Nm ) ranged from 1.27 to 1.56 among the three populations analysed. The multilocus linkage disequilibrium ( LD ) (r ¯d= 0.076–0.117) was moderate in C sativus populations. Cluster analyses indicate that C. sativus populations differentiated according to the hosts (barley and wheat) and tissues (root and leaf) although generalists also exist in North Dakota. Crop breeding may benefit from combining genes for resistance against both specialists and generalists of C. sativus .