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Detection and Identification of 16SrII Group Phytoplasmas Infecting Stylosanthes in China
Author(s) -
Che Haiyan,
Li Zhengnan,
Zhang Lei,
Wu Yunfeng,
Luo Daquan
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2012.01923.x
Subject(s) - biology , stylosanthes , restriction fragment length polymorphism , 16s ribosomal rna , phytoplasma , broom , ribosomal rna , nested polymerase chain reaction , capitata , polymerase chain reaction , botany , gene , genetics , ecology , brassica oleracea
In July 2008, Stylosanthes guianensis with witches’‐broom symptoms and Stylosanthes capitata with yellows symptoms were observed in Hainan Province, China. From the symptomatic S. guianensis , the phytoplasma‐specific 16S rRNA gene fragment of 1.4 kb was amplified by a direct PCR, and a fragment of 16S rRNA gene of 1.2 kb was obtained from the symptomatic Stylosanthes capitata by a nested PCR. Sequencing results and NCBI BLASTn analysis showed that the two phytoplasmas belonged to group 16SrII. Polygenetic analysis and virtual RFLP classified the two phytoplasmas into subgroup 16SrII‐A.