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Calla lily chlorotic spot virus from Spider Lily ( Hymenocallis litteralis ) and Tobacco ( Nicotiana tabacum ) in the South‐west of China
Author(s) -
Liu Yating,
Lu Xiuping,
Zhi Long,
Zheng Yuanxian,
Chen Xuejiao,
Xu Yue,
Wu Fajing,
Li Yongzhong
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01873.x
Subject(s) - biology , nicotiana tabacum , botany , genbank , plant virus , virology , ornamental plant , cucumber mosaic virus , thrips , virus , genetics , gene
Leaf samples were collected from four spider lily and tobacco plants with tospovirus‐like symptoms in Yunnan province. The nucleocapsid ORFs of the four isolates were obtained by RT‐PCR, cloning and sequencing of the amplified products. Nucleotide sequences of the obtained amplicons were more than 90% identical to that of Calla lily chlorotic spot virus (CCSV) isolates deposited in the GenBank database. The virus induced systematic leaf necrosis, chlorotic lesions, rugosity and deformation on mechanically inoculated experimental hosts. This is the first report of CCSV in mainland China, and also on spider lily and tobacco.