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Diversity of Fusarium Species Isolated from Weeds and Plant Debris in Croatia
Author(s) -
Postic Jelena,
Cosic Jasenka,
Vrandecic Karolina,
Jurkovic Drazenka,
Saleh Amgad A.,
Leslie John F.
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01863.x
Subject(s) - biology , amplified fragment length polymorphism , fusarium , botany , population , plant disease , weed , genetic diversity , veterinary medicine , microbiology and biotechnology , sociology , medicine , demography
Weeds are alternative hosts of plant pathogens and when colonized may not exhibit disease symptoms. In 2008 and 2009, samples of weeds and plant debris were collected from 12 locations in eastern Croatia, and 300 Fusarium isolates colonizing them were identified. Strains were grouped and identified based on morphology and amplified fragment length polymorphism (AFLP) patterns. Portions of the β‐tubulin and translocation elongation factor 1‐α genes were sequenced from representative strains of each group to confirm the identifications. Fourteen Fusarium species were identified with F. graminearum (20%), F. verticillioides (18%), F. oxysporum (16%), F. subglutinans ( 13%) and F. proliferatum ( 11%) all present as more than 10% of the population. Fusarium acuminatum , F. avenaceum , F. concolor , F. crookwellense ( F. cerealis ), F. equiseti , F. semitectum , F. solani , F. sporotrichioides and F. venenatum, were all present at frequencies < 8%. Our results indicate that economically important Fusarium spp. may be isolated from numerous alternative hosts during the off season and that weeds and plant debris can serve as a reservoir of genetically diverse inoculum.