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Infection of Alstroemeria Plants with Tomato yellow ring virus in Iran
Author(s) -
Beikzadeh Nasser,
Bayat Hossein,
Jafarpour Behrooz,
Rohani Hamid,
Peters Dick,
HassaniMehraban Afshin
Publication year - 2012
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01851.x
Subject(s) - biology , petal , virus , polyclonal antibodies , virology , reverse transcription polymerase chain reaction , amplicon , gene , botany , polymerase chain reaction , plant virus , horticulture , antibody , microbiology and biotechnology , genetics , messenger rna
Alstroemeria cv. Ovation plants with virus‐like necrotic spots and streaks on leaves and petals were observed in greenhouses in Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces, Iran. Samples with virus‐like symptoms reacted positively in enzyme‐linked immunosorbent assay with a polyclonal antibody raised against Tomato yellow ring virus (TYRV) nucleocapsid (N) protein. TYRV‐specific primers were used in a reverse transcription‐polymerase chain reaction to amplify the N gene. The deduced amino acid sequences of the obtained amplicon revealed 99% identity to the N protein of an isolate of TYRV isolated from tomato (TYRV‐t).