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Grapevine Fleck Virus Isolates Split into Two Distinct Molecular Groups
Author(s) -
Glasa Miroslav,
Predajňa Lukas,
Komínek Petr
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01845.x
Subject(s) - biology , capsid , phylogenetic tree , genome , genetics , gene , virus , virology , divergence (linguistics) , linguistics , philosophy
Although Grapevine fleck virus (GFkV) has a worldwide distribution, data about its molecular variability are very scarce. A genome region encoding the central part of the capsid protein gene was amplified from 36 GFkV isolates recovered from a relatively restricted area (Slovakia and the Czech Republic). The nucleotide identities between isolates ranged from 88 to 100%. Phylogenetic analysis showed that the GFkV isolates divided into two groups. Although group I comprised the majority of the isolates, the analysis of the mean nucleotide intragroup divergence showed that group I was less variable than group II. The d N : d S ratio was <1 for each group, suggesting a negative selective pressure for amino acid changes in this portion of the genome.