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First Report of an Aster Yellows Phytoplasma as the Cause of Rose Balsam Phyllody in China
Author(s) -
Li Zhengnan,
Zhang Lei,
Liu Ping,
Bai Yaobo,
Wu Yunfeng
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01843.x
Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , phyllody , aster yellows , nested polymerase chain reaction , 16s ribosomal rna , botany , amplicon , polymerase chain reaction , genetics , gene
In July 2010, symptoms suggestive of phytoplasma infection were observed on Rose Balsam ( Impatiens balsamina ) around Yangling, China. Nested polymerase chain reaction with universal 16S rDNA phytoplasma primers P1/P6 and R16F2n/R16R2 yielded amplicons of expected size (1.2 kb) from all symptomatic, but not asymptomatic, leaf samples. Sequencing results and NCBI BLASTn analysis of the 1246 bp products (R16F2n/R16R2) showed that the phytoplasma belonged to group 16SrI. Restriction fragment length polymorphism and phylogenetic analysis showed the phytoplasma had a close relationship to subgroup 16SrI‐D. This is the first report of a phytoplasma infecting Rose Balsam.