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Selection of a Suitable Medium to Determine Sensitivity of Monilinia fructicola Mycelium to SDHI Fungicides
Author(s) -
Hu MengJun,
Luo ChaoXi,
Grabke Anja,
Schnabel Guido
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01815.x
Subject(s) - monilinia fructicola , mycelium , fungicide , preharvest , postharvest , horticulture , biology
Succinate dehydrogenase inhibitor (SDHI) fungicides are important tools to control preharvest and postharvest brown rot of stone fruits. In this study we determined the mycelial growth rate of Monilinia fructicola isolates on various media and picked the three that allowed the fastest growth to assess the sensitivity of mycelium to SDHI fungicides boscalid, fluopyram, and penthiopyrad. Minimal medium (MM) supported mycelial growth the best and yielded lowest EC 50 values for three SDHI fungicides. EC 50 values corresponded with disease incidence data obtained from detached fruit assays. Penthiopyrad had significantly greater intrinsic activity in vitro compared to fluopyram at the α = 0.05 level and compared to boscalid at the α = 0.1 level. However, detached fruit assays revealed that this ‘advantage’ did not carry over in vivo . In conclusion, MM appears to be the best medium currently available to assess the sensitivity of M. fructicola mycelium in vitro .