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Characterization of Catharanthus roseus Genes Regulated Differentially by Peanut Witches’ Broom Phytoplasma Infection
Author(s) -
Chen WuuYang,
Lin ChanPin
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2011.01796.x
Subject(s) - biology , catharanthus roseus , gene , phytoplasma , complementary dna , genetics , homology (biology) , cdna library , microbiology and biotechnology , gene expression , gene family , botany , polymerase chain reaction , restriction fragment length polymorphism
The differential display (DD) strategy was applied to isolate periwinkle ( Catharanthus roseus ) cDNAs that were differentially expressed following infection with peanut witches’ broom (PnWB) phytoplasma. Sixty‐four clones were selected from differentially expressed cDNA fragments. Following screening by reverse Northern hybridization, ten transcripts were selected and sequenced. The expression level of each transcript was quantified by real‐time PCR, and seven DD transcripts were identified as truly differentially expressed following PnWB phytoplasma infection. Among these, one that was homologous with phi‐1 gene was up‐regulated, while the others were down‐regulated. Except two genes, other four down‐regulated genes shared homology with the genes encoding psaDa gene, ML domain protein gene, eukaryotic translation initiation factor SUI1 gene and plastidic aldolase NPALDP1 gene, respectively. The identities of homologous genes were further confirmed for three DD transcripts by isolating long cDNA fragments from the cDNA library that was established in this investigation. Verified genes were ML domain protein gene, translation initiation factor SUI1 gene and plastidic aldolase gene and were primarily involved in the innate immune response, the stress response and photosynthesis. The possible role of these genes in the periwinkle that was infected by PnWB phytoplasma is discussed.