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Molecular Characterization of Phytoplasmas Associated with Potato Purple Top Disease in Iran
Author(s) -
Hosseini Parham,
Bahar Masoud,
Madani Golnoush,
Zirak Leila
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2010.01757.x
Subject(s) - biology , disease , botany , phytoplasma , genetics , polymerase chain reaction , gene , restriction fragment length polymorphism , medicine , pathology
Potato plants with symptoms suggestive of potato purple top disease (PPTD) occurred in the central, western and north‐western regions of Iran. Polymerase chain reaction (PCR) and nested PCR assays were performed using phytoplasma universal primer pair P1/P7 followed by primer pairs R16F2n/R16R2 and fU5/rU3 for phytoplasma detection. Using primer pairs R16F2n/R16R2 and fU5/rU3 in nested PCR, the expected fragments were amplified from 53% of symptomatic potatoes. Restriction fragment length polymorphism (RFLP) analysis using Alu I, Cfo I, Eco RI, Kpn I, Hin dIII, Mse I, Rsa I and Taq I restriction enzymes confirmed that different phytoplasma isolates caused PPTD in several Iranian potato‐growing areas. Sequences analysis of partial 16S rRNA gene amplified by nested PCR indicated that ‘ Candidatus Phytoplasma solani’, ‘ Ca . Phytoplasma astris’ and ‘ Ca . Phytoplasma trifolii’ are prevalent in potato plants showing PPTD symptoms in the production areas of central, western and north‐western regions of Iran, although ‘ Ca . Phytoplasma solani’ is more prevalent than other phytoplasmas. This is the first report of phytoplasmas related to ‘ Ca . Phytoplasma astris’, ‘ Ca . Phytoplasma solani’ and ‘ Ca . Phytoplasma trifolii’ causing PPTD in Iran.