Premium
Association of Elm Yellows Subgroup 16SrV‐B Phytoplasma with a Disease of Hovenia dulcis
Author(s) -
KamalaKannan Seralathan,
Han SangSub,
Lee KuiJae,
Velmurugan Palanivel,
Lee Yong Hoon,
Chae JongChan,
Lee YangSoo,
Lee JaiYoung,
Oh ByungTaek
Publication year - 2011
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2010.01746.x
Subject(s) - phytoplasma , biology , broom , 16s ribosomal rna , phylogenetic tree , ribosomal dna , restriction fragment length polymorphism , botany , ribosomal rna , sequence analysis , gene , genetics , polymerase chain reaction , ecology
Japanese raisin ( Hovenia dulcis ) trees with typical phytoplasma‐like symptoms were observed for the first time in South Korea. The disease, named Japanese raisin witches’ broom, is progressively destructive. The cause of the graft‐transmissible disease was confirmed by electron microscopy and molecular studies. The 16S rDNA sequence analysis showed that the phytoplasma was closely related to the elm yellows (EY) group, ribosomal subgroup 16SrV‐B. The 16S‐23S rDNA intergenic spacer region, fragment of rp operon and secY gene sequences had 96–99% similarity with members of EY phytoplasma. Based on the sequence analyses and phylogenetic studies, it was confirmed that the phytoplasma infecting Japanese raisin trees in Korea belongs to the EY group.