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Detection and Identification of a Phytoplasma Related to the 16SrV Group Infecting Chinese Pink in China
Author(s) -
Zhang Chunping,
Min Hong,
Li Zhengnan,
Wu Zhanmin,
Yang Yang,
Wu Yunfeng
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2010.01700.x
Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , phylogenetic tree , nested polymerase chain reaction , polymerase chain reaction , primer (cosmetics) , genetics , gene , chemistry , organic chemistry
Using universal phytoplasma primer pairs R16mF2/R16mR1 and R16F2n/R16R2, nested polymerase chain reactions were used for phytoplasma detection in Chinese pink with white leaves and stunt symptoms. Sequencing results and restriction fragment length polymorphism (RFLP) analysis of the 1248‐bp R16F2n/R16R2 products revealed the phytoplasma belongs to group 16SrV. Phylogenetic analysis showed that the phytoplasma had a close relation to Jujube Witches‐broom phytoplasma. This is the first report of a 16SrV‐related phytoplasma infecting Chinese pink in China.