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Genetic Diversity and Pathogenicity Analysis of Sphaceloma ampelinum Causing Grape Anthracnose in Thailand
Author(s) -
Poolsawat Oythip,
Tharapreuksapong Akkawat,
Wongkaew Sopone,
Reisch Bruce,
Tantasawat Piyada
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2010.01696.x
Subject(s) - biology , pathogenicity , genetic diversity , cultivar , genotype , botany , inoculation , veterinary medicine , horticulture , vitis vinifera , fungi imperfecti , genetic analysis , microbiology and biotechnology , genetics , gene , medicine , population , demography , sociology
Anthracnose is one of the major diseases affecting grape ( Vitis vinifera L.) cultivars in Thailand. Isolates of Sphaceloma ampelinum , the anamorph stage of Elsinoe ampelina , were collected from various regions of Thailand. Nineteen single‐conidial isolates were evaluated for differences in conidial morphology, DNA patterns and pathogenicity. These isolates could not be unambiguously distinguished based on conidial morphology; however, they were genetically differentiated using random amplified polymorphic DNA markers. Cluster analysis by the unweighted paired grouped mean arithmetic average classified these isolates into four groups. Pathogenicity analysis using nine grape genotypes and five S. ampelinum isolates showed that ‘Wilcox321’ and ‘Illinois547‐1 were highly resistant to all isolates, suggesting their usefulness as resistant sources in future breeding programmes.