Premium
Molecular Characterization of Tomato Leaf Curl Hainan Virus, a New Begomovirus , and Evidence for Recombination
Author(s) -
Zhang Hui,
Hu Gaojie,
Zhou Xueping
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2010.01689.x
Subject(s) - begomovirus , leaf curl , biology , geminiviridae , virus , virology , nucleic acid sequence , genome , gene , recombination , plant virus , genetics , tomato yellow leaf curl virus
Virus isolates HaNHK7 and HaNHK8 were obtained from tomato plants showing leaf curl symptoms in Hainan Province of China. The complete DNA sequences of both isolates were determined to be 2748 nucleotides, with all the characteristic features of begomovirus genome organization. The two isolates share 99.8% identity with each other but have <88.3% nucleotide sequence identity with other begomoviruses. Consequently, HaNHK7 and HaNHK8 are considered to be isolates of a novel Begomovirus species, for which the name Tomato leaf curl Hainan virus (ToLCHnV) is proposed. ToLCHnV is most closely related to Papaya leaf curl China virus (PaLCuCNV, AJ558117 ) (88.3% in total nucleotide sequences). However, the AC2 gene more resembles that of Ageratum leaf curl virus (ALCuV, AJ851005 ) and AC1 and AC4 genes resemble those of Tomato leaf curl Vietnam virus (ToLCVV, AF264063 ). Sequence analyses suggest that ToLCHnV may have arisen by recombination between viruses related to PaLCuCNV, ALCuV and ToLCVV. Neither the DNA‐B component nor the DNA‐β molecule was found to be associated with ToLCHnV isolates.