Premium
Genetic Variability Analysis and Molecular Detection of Fusarium oxysporum f.sp. eustomae Isolated from Eustoma grandiflorum in Northern Italy
Author(s) -
Li Yuan,
Garibaldi Angelo,
Gullino Maria Lodovica
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01664.x
Subject(s) - biology , rapd , fusarium oxysporum , pathogen , genetic diversity , fusarium wilt , genetic marker , genetic variability , botany , genetic analysis , fusarium , horticulture , genotype , genetics , gene , population , demography , sociology
A total of 35 isolates of Fusarium oxysporum f.sp. eustomae obtained from diseased Eustoma grandiflorum plants in northern Italy, showing typical Fusarium wilt symptoms, were analysed for their genetic variability and molecular identification. Genetic diversity of the isolates was studied by using random amplified polymorphic DNA (RAPD). This analysis clustered the isolates into three groups at a genetic similarity of 69%. Sequence analysis of RAPD fragments led to the design of a pair of specific primers that amplify a 505‐bp SCAR (sequence characterized amplified region) marker (SCAR 505 ) which was used to rapidly detect F . oxysporum f.sp. eustomae on Eustoma grandiflorum plants. In a temperature‐controlled chamber, detection of the pathogen by PCR was 100% successful in root and stem samples of infected but still symptomless plants. The diagnostic procedure could be completed in 1 day and allowed rapid and reliable detection of the pathogen in asymptomatic plants in the early stages of disease development.