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HpRNAs Derived from Different Regions of the NIb Gene Have Different Abilities to Protect Tobacco from Infection with Potato Virus Y
Author(s) -
Sun ZhaoNan,
Song YunZhi,
Yin GuoHua,
Zhu ChangXiang,
Wen FuJiang
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01650.x
Subject(s) - biology , rna silencing , rna interference , rna , potato virus y , gene , small hairpin rna , tobacco mosaic virus , gene expression , virology , blot , messenger rna , gene silencing , plant virus , microbiology and biotechnology , virus , genetics
RNA interference (RNAi) involves the degradation of homologous mRNA sequences in organisms and is induced by double‐stranded RNA (dsRNA). We have constructed three hairpin RNA (hpRNA) prokaryotic expression vectors derived from the 5′ region, the middle region and the 3′ region of the Nuclear inclusion protein b ( NIb ) gene, and then expressed these in M‐JM109lacY. Resistance analyses and Northern blotting showed that different hpRNAs had different abilities to protect tobacco plants from infection with Potato virus Y (PVY) and that this resistance was RNA‐mediated.