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Control of Plant Pathogenic Bacteria of Xanthomonas spp. by the Essential Oil and Extracts of Metasequoia glyptostroboides Miki ex Hu In vitro and In vivo
Author(s) -
Bajpai Vivek K.,
Cho Moon Jae,
Kang Sun Chul
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01646.x
Subject(s) - biology , xanthomonas campestris , xanthomonas , microbiology and biotechnology , antimicrobial , pathogenic bacteria , minimum inhibitory concentration , bacteria , horticulture , botany , food science , genetics
Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and non‐biodegradable and cause extended environmental pollution. Therefore, this study was undertaken to assess the in vitro and in vivo antibacterial efficacy of the essential oil and organic extracts of Metasequoia glyptostroboides against plant pathogenic bacteria of Xanthomonas spp. The oil (1000 μg/disc) and extracts (1500 μg/disc) displayed potential antibacterial effect in vitro as a diameter of zones of inhibition against Xanthomonas campestris pv. campestris KC94‐17‐XCC, X. campestris pv. vesicatoria YK93‐4‐XCV, X. oryzae pv. oryzae KX019‐XCO and X. sp SK12, which were found in the range of 10–14 and 8–12 mm, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of oil and the extracts were ranged from 125–250 and 125 500 μg/ml and 250–1000 and 250–2000 μg/ml, respectively. Also the oil had strong detrimental effect on the viable count of the tested bacteria. Further, the oil displayed remarkable in vivo antibacterial effect up to 65 to 100% disease suppression efficacy against the tested strains of Xanthomonas spp. on greenhouse‐grown oriental melon plants ( Cucumis melo L. var. makuwa ). These results suggest that the oil and extracts of M. glyptostroboides could be potential source of natural antibacterials for applying in food and agriculture industries to control plant bacterial diseases caused by Xanthomonas spp.