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Use of Rep‐PCR for Genetic Diversity Analyses in Fusarium culmorum
Author(s) -
Gurel Filiz,
Albayrak Gulruh,
Diken Ozlem,
Cepni Elif,
Tunali Berna
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01630.x
Subject(s) - fusarium culmorum , biology , genetic diversity , dendrogram , intergenic region , genetics , similarity (geometry) , strain (injury) , genomic dna , fusarium , genome , gene , population , demography , image (mathematics) , anatomy , artificial intelligence , sociology , computer science
Abstract Fusarium culmorum is a pathogen of economically important grain crops. In this work, Rep‐PCR was used to identify genetic diversity in F. culmorum isolates which have been collected from wheat fields in Turkey. Reproducible genomic fingerprints were amplified in each strain by PCRs of prokaryotic repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC) and BOX sequences. Totally 104 molecular markers were evaluated and similarity comparisons were shown as a dendrogram. The average genetic diversity was 52.3% ranging from 15.8% to 88.7% according to the Rep‐PCR data. Cluster analysis showed agreement with the distance of sampling locations. The highest genetic similarity (84.2%) was determined between two F. culmorum isolates (F1 and F2) originated from the same agro‐ecological region. Our results showed that Rep‐PCR is convenient and rapid for genetic diversity analyses and strain differentiation in F. culmorum .