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Development of New Oomycete Taxon‐specific Mitochondrial Cytochrome b Region Primers for Use in Phylogenetic and Phylogeographic Studies
Author(s) -
Giresse Xavier,
Ahmed Sophia,
RichardCervera Sylvie,
Delmotte François
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01619.x
Subject(s) - oomycete , biology , phylogenetic tree , phylogeography , taxon , cytochrome b , evolutionary biology , mitochondrial dna , genetics , phylogenetics , population , primer (cosmetics) , botany , gene , chemistry , demography , organic chemistry , sociology
Here, we describe the development of an oomycete‐specific primer pair for amplification of the cytochrome b region in plant pathogenic species that span the order Peronosporales ( Phytophthora spp., downy mildews). Because of the high number of variable sites at both inter‐ and intra‐specific levels this marker provides a powerful tool for population genetics and phylogenetic studies in this taxa. We also demonstrate its potential compared with other oomycete‐specific mitochondrial markers currently available.