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In vitro Antibiosis by Pseudomonas syringae Pss22d, Acting Against the Bacterial Blight Pathogen of Soybean Plants, Does Not Influence In planta Biocontrol
Author(s) -
Braun Sascha D.,
Hofmann Janine,
Wensing Annette,
Weingart Helge,
Ullrich Matthias S.,
Spiteller Dieter,
Völksch Beate
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01612.x
Subject(s) - biology , pseudomonas syringae , antibiosis , pathogen , microbiology and biotechnology , antagonism , biological pest control , pseudomonadaceae , pseudomonadales , toxin , pseudomonas , coronatine , botany , mutant , bacteria , arabidopsis , gene , biochemistry , genetics , receptor
The epiphyte Pseudomonas syringae pv. syringae 22d / 93 (Pss22d), isolated from soybean leaves, had been characterized as a promising and species‐specific biocontrol strain in vitro and in planta against the plant pathogen P. syringae pv. glycinea (Psg), which causes bacterial blight of soybean. Three toxins are known to be produced by Pss22d: syringomycin, syringopeptin and 3‐methylarginine (MeArg). In contrast to syringopeptin and syringomycin, MeArg inhibited the growth of Psg in vitro . To examine if the toxins produced by Pss22d are responsible for antagonistic effects in planta , the pathogen Psg was co‐inoculated with either Pss22d wild‐type, a syringopeptin/syringomycin‐negative double mutant (Pss22d.ΔsypA/syrE), or a MeArg‐negative mutant (Pss22d.1) into wounds of pin‐pricked leaves of greenhouse‐grown soybean plants, respectively. In all three cases, the wild‐type Pss22d and its toxin‐deficient mutants prevented development of disease symptoms normally caused by Psg. These results indicated that neither syringopeptin, nor syringomycin, nor MeArg was required for Pss22d’s antagonistic activity in planta . Consequently, factors other than the three toxins may contribute to the intra‐species antagonism in planta .