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First Report of Aster Yellows Phytoplasma in Goldenrain Tree ( Koelreuteria paniculata ) in Korea
Author(s) -
KamalaKannan Seralathan,
Park SeungMoon,
OH ByungTaek,
Kim HyungMoo,
Lee KuiJae
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01602.x
Subject(s) - phytoplasma , biology , aster yellows , phylogenetic tree , 16s ribosomal rna , botany , gene , polymerase chain reaction , restriction fragment length polymorphism , genetics
Abstract Aster yellows phytoplasma was detected for the first time in goldenrain tree ( Koelreuteria paniculata ) growing in Sinpyeong‐myeon, Jeollabuk‐do, South Korea. DNA was extracted from the infected leaf samples and part of the 16S rDNA, rp operon and tuf gene were amplified using R16F2n/R2 and gene‐specific primers. The sequence analysis showed that the phytoplasma was closely related (99%) to members of the Aster Yellows (AY) group, and belonging to 16Sr I, subgroup B. Moreover, the 16S rDNA sequences of the isolate showed 88–96% identity with members of other 16Sr and undesignated groups. Based on the sequence identity and phylogenetic studies, it was confirmed that phytoplasma infecting goldenrain tree in South Korea belongs to the AY group.