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Pectolytic Bacteria Associated with Soft Rot of Calla Lily ( Zantedeschia spp.) Tubers
Author(s) -
Mikiciński Artur,
Sobiczewski Piotr,
Sulikowska Monika,
Puławska Joanna,
Treder Jadwiga
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01597.x
Subject(s) - pectobacterium carotovorum , biology , microbiology and biotechnology , bacteria , pectobacterium , calla , inoculation , pathogen , pectate lyase , erwinia , pectinase , horticulture , biochemistry , genetics , immunology , antibody , monoclonal antibody , enzyme
Soft rot is the most important disease on calla lily in Poland. The isolation of the presumptive pathogen from symptomatic tubers on nutrient agar yielded bacteria with different colony morphology. Of 41 isolates collected, 10 showed pectolytic activity on crystal violet pectate medium and caused soft rot on potato slices. All pectolytic bacteria appeared to be Gram‐negative rods producing typical soft rot on inoculated leaf petioles of calla lily. Bacteria with colonies which morphologically resembled those used for inoculation were re‐isolated from diseased petioles. Their identification was based on phenotypic characters and sequence of the gene fragment coding 16S rRNA. It was found that, in addition to Pectobacterium carotovorum subsp. carotovorum , soft rot of calla lily can be caused by Pectobacterium carotovorum subsp . atrosepticum , Pseudomonas marginalis , Pseudomonas veronii and Chryseobacterium indologenes . The latter two are described for the first time as plant pathogens. The pectolytic activity of all identified bacteria, except that of P. carotovorum subsp . atrosepticum , was lower than that of P. carotovorum subsp. carotovorum , but strains of P. veronii showed a higher activity than P. marginalis and C. indologenes species.