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Specific Isolation of RNA from the Grape Powdery Mildew Pathogen Erysiphe necator , an Epiphytic, Obligate Parasite
Author(s) -
CadleDavidson Lance,
Wakefield Laura,
Seem Robert C.,
Gadoury David M.
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01578.x
Subject(s) - powdery mildew , biology , conidium , botany , obligate parasite , appressorium , hypha , epiphyte , microbiology and biotechnology , host (biology) , genetics
RNA expression profiling of obligately parasitic plant microbes is hampered by the requisite interaction of host and parasite. This can be especially problematic in the case of powdery mildews, such as Erysiphe necator (syn. Uncinula necator ), which grow superficially but tightly adhere to the plant epidermis. We developed and refined a simple and efficient technique in which nail polish was used to remove conidia, appressoria, hyphae, conidiophores, and developing ascocarps of E. necator from grapevine ( Vitis vinifera ) leaves and showed that RNA isolated after removal was not contaminated with V. vinifera RNA. This approach can be applied to expression analyses throughout fungal development and could be extended to other epiphytic pathogens and saprophytes.