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Characterization of Phytoplasmas Related to ‘ Candidatus Phytoplasma asteris’ and Peanut WB Group Associated With Sweet Cherry Diseases in Iran
Author(s) -
Zirak Leila,
Bahar Masoud,
Ahoonmanesh Ali
Publication year - 2010
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01566.x
Subject(s) - phytoplasma , biology , 16s ribosomal rna , restriction fragment length polymorphism , nested polymerase chain reaction , mollicutes , intergenic region , polymerase chain reaction , botany , gene , genetics , bacteria , genome
Symptoms suggestive of phytoplasma diseases were observed in infected sweet cherry trees growing in the central regions of Iran. Phytoplasmas were detected in symptomatic trees by the nested polymerase chain reaction (nested PCR) using phytoplasma universal primer pairs (P1/Tint, PA2F/R, R16F2/R2 and NPA2F/R). Restriction fragment length polymorphism analyses of 485 bp DNA fragments amplified in nested PCR revealed that different phytoplamas were associated with infected trees. Sequence analyses of phytoplasma 16S rRNA gene and 16S‐23S intergenic spacer region indicated that the phytoplasmas related to ‘ Ca. Phytoplasma asteris ’ and peanut WB group infect sweet cherry trees in these regions. This is the first report of the presence of phytoplasmas related to ‘ Ca. Phytoplasma asteris’ and peanut WB group in sweet cherry trees.