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Detection of Sclerotinia sclerotiorum in Planta by a Real‐time PCR Assay
Author(s) -
Yin Yanni,
Ding Laisong,
Liu Xin,
Yang Jinghui,
Ma Zhonghua
Publication year - 2009
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2009.01543.x
Subject(s) - sclerotinia sclerotiorum , biology , primer (cosmetics) , sclerotinia , polymerase chain reaction , real time polymerase chain reaction , stem rot , fungus , horticulture , genetics , botany , gene , chemistry , organic chemistry
Stem rot caused by Sclerotinia sclerotiorum is a very serious disease on oilseed rape worldwide. In this study, a pair of polymerase chain reaction (PCR) primers was designed based on the nucleotide sequence of a DNA region amplified by a microsatellite primer M13. The primer pair amplified a 252‐bp fragment from all S. sclerotiorum isolates collected from oilseed rapes at different locations in different years, but not from any other fungus tested. Using this pair of primers, a real‐time PCR assay was developed to rapidly detect early infection of S. sclerotiorum on petals of oilseed rape. The real‐time PCR assay developed in this study could help growers make a timely decision on fungicide application.