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Identification of Coffee Genes Expressed During Systemic Acquired Resistance and Incompatible Interaction with Hemileia vastatrix
Author(s) -
Guzzo Sylvia Dias,
Harakava Ricardo,
Tsai Siu Mui
Publication year - 2009
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2008.01538.x
Subject(s) - biology , suppression subtractive hybridization , gene , complementary dna , systemic acquired resistance , hypersensitive response , cdna library , expressed sequence tag , genetics , gene expression , microbiology and biotechnology , plant disease resistance , arabidopsis , mutant
Coffee genes associated with systemic acquired resistance (SAR) and incompatible reaction against coffee leaf rust inoculation were identified by suppression subtractive hybridization. Analysis of 384 clones of each of the subtracted cDNA libraries identified genes involved in oxidative burst/apoptosis/hypersensitive response, synthesis of antimicrobial proteins, synthesis and transport of antimicrobial metabolites, signal perception and transduction, metabolism of lipids, regulated protein degradation and cell maintenance and development. Induction of distinct sets of genes in the two resistance responses was observed. A wide range of genes involved in defence responses described in other plant species was also found in coffee plants. Semi‐quantitative and quantitative RT‐PCR analysis of seven selected genes showed differences in their expression profile within 72 h after treatment. Full‐length cDNA sequences of two β‐1,3‐glucanases, one induced during SAR and the other in the incompatible reaction, were obtained by 5′ and 3′ RACE and the sequence data suggest different properties and cellular localization of the encoded proteins.