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Immunodiagnosis of Parietaria mottle virus in Tomato Crops Using a Polyclonal Antiserum against its Coat Protein Expressed in a Bacterial System
Author(s) -
Aparicio Frederic,
Aramburu José,
Soler Salvador,
Galipienso Luis,
Nuez Fernando,
Pallás Vicente,
López Carmelo
Publication year - 2009
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2008.01511.x
Subject(s) - polyclonal antibodies , antiserum , biology , recombinant dna , fusion protein , immunoassay , affinity chromatography , escherichia coli , virology , microbiology and biotechnology , antigen , antibody , gene , enzyme , biochemistry , genetics , immunology
Recombinant DNA technology was used to raise a polyclonal antiserum against the coat protein (CP) of Parietaria mottle virus (PMoV). The CP gene was expressed in Escherichia coli as a fusion to a 6xHis tag and purified by affinity chromatography. Recombinant purified protein was used as antigen to raise a polyclonal antiserum. This polyclonal antiserum consistently detected PMoV specifically infected tomato plants from different commercial tomato crops by indirect enzyme‐linked immunosorbent assay (I‐ELISA) and direct tissue‐printing immunoassay (DTBIA).