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Differentiation and Genetic Structure of Sclerophoma pythiophila Strains on Pinus sylvestris in Poland
Author(s) -
Kraj Wojciech
Publication year - 2009
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2008.01497.x
Subject(s) - jaccard index , biology , pinus <genus> , microsatellite , analysis of molecular variance , genetic variability , genetic variation , strain (injury) , genetic structure , genetic similarity , genetic diversity , similarity (geometry) , botany , veterinary medicine , genetics , genotype , allele , statistics , demography , gene , population , anatomy , mathematics , artificial intelligence , image (mathematics) , sociology , computer science , cluster analysis , medicine
Between 1996 and 2006, 97 strains of Sclerophoma pythiophila were isolated from 3 to 10‐year‐old trees of Pinus sylvestris from three regions of Poland differing by climatic conditions and geographic location. On the basis of 56 random amplified microsatellites (RAMS) markers obtained with the use of five primers, very high level of genetic variability of strains (mean Jaccard’s coefficient 0.56) was ascertained. Among the analysed markers only one was completely monomorphic, whereas six were monomorphic in 90%. Greater genetic similarity was ascertained for strains in southern Poland (0.58) in comparison with strains deriving from northern Poland (0.52). Correlation between the percentage share of polymorphic loci and the average annual temperature of places of strain isolation ( r = 0.62) were shown, as well as the number of days with snow cover ( r = 0.79). On the basis of the amova analysis, it was ascertained that 11.6% of genetic variability was located between regions of the strains origin and 88.4% within the regions.