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Effect of a Photo‐synthetic Inhibitor on Tryptamine Pathway‐mediated Sekiguchi Lesion Formation in Lesion Mimic Mutant of Rice Infected with Magnaporthe grisea
Author(s) -
Imaoka A.,
Ueno M.,
Kihara J.,
Kadowaki M.,
Arase S.
Publication year - 2008
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2008.01405.x
Subject(s) - magnaporthe grisea , tryptamine , dcmu , biology , mutant , tryptophan , catalase , biochemistry , microbiology and biotechnology , enzyme , chloroplast , oryza sativa , gene , amino acid
A lesion‐mimic mutant of rice (cv. Sekiguchi‐asahi) showed enhanced resistance to Magnaporthe grisea infection, thereby inducing Sekiguchi lesion ( sl ) formation and tryptamine accumulation under light. Both Sekiguchi lesion formation and tryptamine accumulation in leaves infected with M. grisea were inhibited by pretreatment with the photosynthetic inhibitor, 3‐(3, 4‐Dichlorophenyl)‐1,1‐dimethylurea (DCMU), which suppressed the gene expression of tryptophan decarboxylase ( TDC ), monoamine oxidase activity, H 2 O 2 generation and DNA fragmentation. Catalase activity was inhibited by M. grisea infection under light, but magnitude of the inhibition was reduced in leaves pretreated with DCMU. Furthermore, tryptophan accumulated in M. grisea‐ infected leaves under light but not in DCMU‐pretreated ones. Interestingly, such DCMU inhibition was reduced in the presence of tryptophan. Our studies suggest that chloroplasts function as the inhibitor of anti‐oxidant system such as catalase activity and the supplier of a precursor of tryptamine and tryptophan in the sl mutant infected with M. grisea .