Premium
Whole Genome Sequence and Characterization of a Novel Isolate of PVY Inducing Tuber Necrotic Ringspot in Potato and Leaf Mosaic in Tobacco
Author(s) -
Chikh Ali M.,
Maoka T.,
Natsuaki K. T.
Publication year - 2008
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2007.01377.x
Subject(s) - potato virus y , biology , virology , potyvirus , phylogenetic tree , genome , potyviridae , gene , tobacco mosaic virus , tobacco etch virus , recombinant dna , genetics , virus , plant virus
In a previous study on a Syrian isolate of Potato virus Y (PVY), namely PVY‐12, a point mutation in the coat protein (CP) was detected. This mutation caused the double reactivity of this isolate to monoclonal antibodies specific to O and N serotypes. We report here the biological and molecular characteristics of PVY‐12. In potato, PVY‐12 behaved like a PVY NTN isolate inducing potato tuber necrotic ringspot disease although it induced mosaic in tobacco like PVY O . The genomic analysis grouped PVY‐12 with the recombinant PVY NTN isolates, which is consistent with the phenotype in potato. PVY‐12 HC‐Pro had the two amino acids K400 and E419 that were previously reported as determinant keys of the tobacco necrotic response. This indicates the involvement of other determinants in this phenotype yet to be determined. This is the first report on a PVY NTN isolate that induces mosaic in tobacco, implying that the induction of potato tuber necrosis does not require the ability to induce the tobacco necrosis. PVY‐12 genome had four recombinant points in the P1, HC‐Pro/P3, 6K2/NIa and C terminal region of the CP gene identical to those of PVY NTN isolates 12–94 and 34/01. The PVY‐12 central genomic part flanked by nucleotide positions 2414 and 8604 had highest similarity with that of the Syrian isolate SYR‐NB‐16 suggesting a common origin of these isolates. This common origin was supported using the phylogenetic analysis of this region. In addition, the phylogenetic analysis of the whole genome of the reported North American PVY N:O and the European PVY N W along with other PVY isolates suggests that PVY N:O might have descended from PVY N W with the isolate SASA‐207 as a nearest‐known relative.