Premium
Estimation of Different Cellular Compounds from the Mycelium of Phomopsis theae , Causal Agent of Phomopsis Canker Disease of Tea
Author(s) -
Ponmurugan P.,
Baby U. I.
Publication year - 2008
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2007.01366.x
Subject(s) - mycelium , biology , phomopsis , amino acid , sugar , botany , fungi imperfecti , biochemistry
Variations between three isolates (MP, TP and WP) of Phomopsis theae were studied based on the growth pattern, cellular constituents in the mycelium and protein expression profile. The growth initially increased and later decreased. The growth of these isolates in terms of mycelial dry weight exhibited in the order of MP > TP > WP isolates. The quantitative estimation of cellular constituents such as total sugar, reducing sugar, non‐reducing sugar, nitrogen, protein, amino acids, polyphenol, catechin and lipid showed that there was no significant difference in these constituents between the isolates. The detection of amino acids in the mycelium of P. theae isolates showed 16 free forms and 11 bound forms. Amino acids in the bound form were elucidated to be higher in all the isolates than in the free form. All the isolates of P. theae exhibited prominent protein pattern with three common bands, which lies between the molecular weight of 116 and 66.2 kDa.