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Partial Molecular Characterization of Some Grapevine fanleaf virus Isolates from North‐east of Iran
Author(s) -
Pourrahim R.,
Farzadfar S. H.,
Golnaraghi A. R.,
Ahoonmanesh A.
Publication year - 2007
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2007.01299.x
Subject(s) - biology , genbank , phylogenetic tree , amplicon , homology (biology) , gene , polymerase chain reaction , genetics , phylogenetics , virology
Five isolates of Grapevine fanleaf virus (GFLV) collected from two viticultural regions in the north‐east of Iran were characterized based on coat protein (CP) gene sequences. The CP gene of all isolates was amplified by reverse transcription‐polymerase chain reaction (RT‐PCR) using its specific primers, yielding a 1500‐bp amplicon. The nucleotide sequences for these isolates were obtained and compared with the CPI region of seven isolates from north‐west Iran and also with some other exotic GFLV‐CP sequences available in the GenBank. Analysis of our data revealed that the north‐east Iranian sequences have 83.7–87.5% of homology with the CPI regions of exotic GFLV isolates, and 83.0–87.1% homology with other isolates from north‐west Iran. High nucleotide homology (98.6–100%) was found among the GFLV isolates sequences obtained in this study. The deduced proteins obtained for our five isolates were 92.0–96.0% identical to other sequences. The generated phylogenetic tree revealed a clustering of GFLV isolates distributed over the world in one branch, and the Iranian isolates in other branches.