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The Occurrence and Characterization of New Recombinant Isolates of PVY Displaying Shared Properties of PVY N W and PVY NTN
Author(s) -
Chikh Ali M.,
Maoka T.,
Natsuaki K. T.
Publication year - 2007
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2007.01251.x
Subject(s) - recombinant dna , potato virus y , biology , virology , potyvirus , serology , genome , monoclonal antibody , gene , virus , antibody , genetics , plant virus
Isolates of Potato virus Y (PVY), collected in Syria, induced vein necrosis in tobacco but reacted to PVY O monoclonal antibody, and were therefore classified as PVY N W (or PVY N:O ). The possible recombinant points within the genome were checked using a previously reported multiplex RT‐PCR. These isolates were categorized in two groups according to recombinant points. Isolates with one recombinant point at the HC‐Pro/P3, identical to previously reported PVY N W (or PVY N:O ), were designated as group 1. However, the majority of Syrian PVY isolates showed two recombinant points at both HC‐Pro/P3 and 6K2/NIa, revealing a new genomic recombination pattern that had never been reported for PVY N W (or PVY N:O ), hereafter referred to as group 2. Further characterizations showed that isolates of group 2 had an additional recombinant point at the N‐terminal of the coat protein gene (CP) of their genome. Based on the bioassay on tobacco, serological and molecular studies, these isolates showed shared characteristics of both PVY N W and the recombinant PVY NTN , making it difficult to include them in any of these variants. We propose them as new isolates of PVY N strain, and label them PVY SYR (SYR is from Syria, the country of origin).