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Detection of a Specific Transposon in Erysiphe necator from Grapevines in France
Author(s) -
Bouscaut J.,
CorioCostet M.F.
Publication year - 2007
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2007.01246.x
Subject(s) - biology , powdery mildew , retrotransposon , necator americanus , genetics , transposable element , gene , restriction fragment length polymorphism , mycovirus , botany , polymerase chain reaction , genome , rna , zoology , rna polymerase , helminths , ascaris lumbricoides
The biotrophic fungus, Erysiphe necator the causal agent of the grape powdery mildew, has two genetic groups A and B in European and Australian vineyards. A strain of group A was used to isolate a DNA sequence that exhibits high sequence homology to RNaseH of a non‐LTR (long tandem repeat) retrotransposon of Glomerella cingulata . PCR primers were designed and tested for their specificity to genetic group A of E. necator . This molecular tool is more efficient and sensitive than nested PCR based on polymorphism in the CYP 51 and β ‐tubulin genes.