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Complete Nucleotide Sequence and Construction of an Infectious Clone of a Tunisian Isolate of Tomato yellow leaf curl Sardinia virus
Author(s) -
Chouchane S. Gharsallah,
Gorsane F.,
Nakhla M. K.,
Maxwell D. P.,
Marrakchi M.,
Fakhfakh H.
Publication year - 2006
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2006.01158.x
Subject(s) - biology , clone (java method) , virus , virology , genetics , leaf curl , whole genome sequencing , genome , nucleic acid sequence , gene , plant virus
The full‐length genome of a Tunisian isolate of Tomato yellow leaf curl Sardinia virus (TYLCSV) was engineered and submitted to sequence analysis. The Tunisian isolate has 99% sequence identity with TYLCSV‐Sicilian (Sic), results thus providing further evidence for the inclusion of this isolate in the TYLCSV‐Sic group. A 1.7‐mer construct of the virus was obtained and efficiently agroinfiltrated into tomato and tobacco plants to induce symptoms indistinguishable from those of natural infection.