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A Suspension Culture Method for the Rapid Mass Culture of Cistella japonica Mycelium
Author(s) -
Yamanobe T.,
Oribe Y.
Publication year - 2006
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2006.01140.x
Subject(s) - bran , mycelium , japonica , biology , mass culture , agar , inoculation , incubation , agar plate , botany , food science , horticulture , bacteria , biochemistry , raw material , ecology , genetics , sociology , anthropology
Different methods were investigated for the rapid mass culture of Cistella japonica by using water extracts of some nutritional sources. In an agar culture test, there was little difference in mycelial growth in water extracts of wheat bran, rice bran and potato. In suspension culture with wheat bran extract, which is easily and cheaply available, the mycelium of C. japonica increased seven times more than that in agar culture after a month's incubation. C. japonica from suspension culture was pathogenic to Chamaecyparis obtusa . These results suggest that suspension culture in water extract of wheat bran can be adopted for the rapid mass culturing of C. japonica for use in inoculation tests.