Premium
Microtiter Method to Test the Sensitivity of Botrytis cinerea to Boscalid
Author(s) -
Stammler G.,
Speakman J.
Publication year - 2006
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2006.01139.x
Subject(s) - botrytis cinerea , biology , microtiter plate , botrytis , botany , horticulture , microbiology and biotechnology
In 2000, 137 strains of Botrytis cinerea were isolated from different plant hosts worldwide. The isolates had not been exposed to boscalid and were therefore suitable to establish a baseline sensitivity. In order to determine the sensitivity of these isolates, an appropriate microtiter assay was developed. YBA‐medium was the medium of choice, as it allowed sufficient growth but did not lead to false positive results. ED50‐values ranged from 0.01 to 0.21 ppm, the ED98‐values from 0.1 to 2.21 ppm. No growth was observed at concentrations >3 ppm.