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A Method for Purifying Cochliobolus sativus Cultures Contaminated with Bacteria
Author(s) -
Arabi M. I. E.,
Jawhar M.
Publication year - 2005
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2005.00999.x
Subject(s) - biology , cochliobolus sativus , bacteria , contamination , agar , agar plate , potato dextrose agar , botany , microbiology and biotechnology , cultivar , ecology , genetics
A simple method was developed for purifying Cochliobolus sativus cultures contaminated with bacteria. In this method the contaminated fungal culture is scraped from the surface of the potato dextrose agar (PDA) growth medium. Small leaf pieces (approximately 0.5 cm) of a highly susceptible genotype of barley were placed on the scraped surface of PDA and incubated for 72 h. Disease symptoms of C. sativus were detected on the leaf pieces. Colonies that developed from these pieces on new PDA medium were free from bacteria.