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The Correlation between Severity of Disease Symptoms and the Accumulation of Viral Antigen and Acidic Pathogenesis‐related Proteins in the Leaves of Thorn‐apple Plants Infected with Different Isolates of Potato Virus X
Author(s) -
Sapotsky M. V.,
Romanova S. A.,
Polyakova A. M.,
Malinovsky V. I.
Publication year - 2005
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2005.00997.x
Subject(s) - biology , datura stramonium , inoculation , virus , pathogenesis , antigen , virology , polyacrylamide gel electrophoresis , solanaceae , potato virus x , plant virus , pathogenesis related protein , disease , microbiology and biotechnology , botany , horticulture , immunology , gene , biochemistry , enzyme , medicine , pathology
The isolates of potato virus X causing disease symptoms of different severity in thorn‐apple plants ( Datura stramonium L.) can be categorized in terms of the increase in antigen accumulation, as follows: strong, medium and weak. Extracts from leaves of healthy plants and of those infected with separate virus isolate did not differ in set of acidic pathogenesis‐related (PR) proteins as shown by discontinuous non‐denaturating polyacrylamide gel electrophoresis. Within 2 weeks after inoculation the total content of PR‐proteins in leaves of plants infected with weak or medium isolates was higher than in the healthy ones but after 3 weeks these differences became negligible. The plants infected with the strong isolate had higher total content of PR‐proteins than healthy plants and plants infected with weak or medium isolates during 3 weeks. It is proposed that the greater the damage to the plant caused by virus infection, the more PR‐proteins are accumulated.