Premium
β ‐1,3‐Glucanase and Chitinase Activities and the Resistance Response of Wheat to Leaf Rust
Author(s) -
AnguelovaMerhar V. S.,
Westhuizen A. J.,
Pretorius Z. A.
Publication year - 2001
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2001.tb03866.x
Subject(s) - chitinase , biology , glucanase , rust (programming language) , hypersensitive response , puccinia recondita , inoculation , poaceae , microbiology and biotechnology , septoria , botany , resistance (ecology) , horticulture , plant disease resistance , enzyme , agronomy , biochemistry , gene , computer science , programming language
To investigate the role of β ‐1,3‐glucanases (EC 3.2.1.39) and chitinases (EC 3.2.1.14) in the resistance response of wheat ( Triticum aestivum L.) to leaf rust ( Puccinia recondita f. sp. tritici ), their intercellular activities were studied in resistant (Karee/ Lr35 ) and susceptible (Karee) near‐isogenic wheat lines under conditions of infection and non‐infection. Resistance was associated with high constitutively expressed chitinase activity and induced β ‐1,3‐glucanase activity. At 168 hours post‐inoculation several infection sites on Karee/ Lr35 displayed necrosis, confirming a hypersensitive response, whereas similar necrotic lesions were observed only for a limited number of infection sites on Karee at that sampling stage. The possible role of chitinases and β ‐1,3‐glucanases in the hypersensitive defence response of wheat to leaf rust is discussed.