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Phylogenetische Erkenntnisse auf Grundlage einer auf Informationstheorie basierenden PCR‐Amplifikation
Author(s) -
Tooley P. W.,
Salvo J. J.,
Schneider T. D.,
Rogan P. K.
Publication year - 1998
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1998.tb04776.x
Subject(s) - biology , oomycete , phylogenetic tree , ribosomal dna , primer (cosmetics) , polymerase chain reaction , magnaporthe grisea , genetics , dna sequencing , ribosomal rna , phylogenetics , phylogenetic network , phytophthora infestans , computational biology , pathogen , dna , gene , chemistry , organic chemistry , oryza sativa
Abstract As a method for the determination of the taxonomic affinities of plant pathogens and other organisms, a set of ‘universal’ polymerase chain reaction (PCR) primers which amplify a taxonomically diverse sequence domain of 28S ribosomal DNA (rDNA) were designed. The PCR primers chosen by information‐theory analysis generated PCR products using DNA templates from a wide diversity of organisms. Sequences of PCR products were then obtained which allowed phylogenetic dendrograms to be constructed. Based on the above analysis, the Oomycete pathogen Phytophthora infestans clustered with the protist Prorocentrum micans rather than with representatives of the true fungi, consistent with its designation as a ‘pseudofungus’, Magnaporthe grisea. another important plant pathogen, clustered with the true fungi as expected. The approach described can be used with other plant pathogens to clarify phytogeny of new or ambiguously designated species.