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Expression und Phosphorylierung von Abutilon‐Mosaik‐Geminivirus‐Proteinen in Escherichia coli
Author(s) -
Wege C.,
Jeske H.
Publication year - 1998
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1998.tb04763.x
Subject(s) - biology , escherichia coli , open reading frame , plasmid , antiserum , expression vector , translation (biology) , microbiology and biotechnology , virology , genetics , gene , messenger rna , recombinant dna , peptide sequence , antigen
In order to get more detailed information on single proteins involved in the life cycle of Abutilon mosaic geminivirus, special conditions were established allowing expression of the proteins encoded by different viral open reading frames (AC2, AC3, AVI, BC1, and BV1) in Escherichia coli by use of the pET expression vector system. These include cotransformation of a tRNA supplementation plasmid to overcome codon usage problems in the case of AC2, Protocols were developed to purify the virus proteins for production of antisera. Phosphorylation of AbMV proteins AC3, AVI, BCl, and BV1 in E. coli could be shown by autoradiographic detection of stably incorporated inorganic 32 PO 3 4 .