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Entwicklung und Anwendung monoklonaler Antikörper gegen Xylella fastidiosa , den Erreger der bakteriellen Blattbräune der Birne
Author(s) -
Leu H. H.,
Leu L. S.,
Lin C. P.
Publication year - 1998
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1998.tb04747.x
Subject(s) - xylella fastidiosa , biology , pear , monoclonal antibody , botany , bacteria , virology , microbiology and biotechnology , antibody , genetics
Ten stable hybridoma cell lines, M As l ‐10, secreting monoclonal antibodies specific to the causal bacterium of pear leaf scorch (PLS), Xylella fastidiosa . were produced. The monoclonal antibodies can detect 3 × 10 5 PLS‐bacterium cells by indirect enzyme‐linked immunosorbent assay (ELISA). In the antibody titer determination by indirect ELISA, hybridoma‐culture supernatant from clone MA4 had the highest titer of 20480. In the antibody specificity tests, nine of the 10 monoclonal antibodies did not cross‐react with 14 other bacterial strains belonging to nine genera. Only the antibody from hybridoma clone MAI cross‐reacted with Xanthomonas campestris pv. cam‐pestris and X. campestris pv. vesicatoria . In western blot analysis, all the monoclonal antibodies recognized the major 46.9‐kDa polypeptide from all 12 X. fastidiosa strains and a distinct 21.5‐kDa polypeptide only from PLS bacterium. In tissue‐blotting detection, the PLS bacteria were specifically detected in blots of tissue sections from infected pear with the antibodies developed.