Premium
Resistenz transgener Tabakpflanzen gegen das Pepper severe mosuc potyvinis
Author(s) -
Rabinowicz P. D.,
BravoAlmonacid F. F.,
Lampasona S.,
Rodriguez F.,
Gracia O.,
Mentaberry A. N.
Publication year - 1998
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1998.tb04698.x
Subject(s) - potyvirus , biology , inoculation , virus , pepper , transgene , genetically modified crops , titer , tobacco mosaic virus , virology , horticulture , plant virus , solanaceae , gene , botany , genetics
The coat protein gene of the pepper severe mosaic poty‐virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon challenge with PepSMV, R 2 descendants from this line were monitored for virus accumulation for a period of 45 days. Virus titre was lower in plants of the line 232 than in control plants throughout the experiment. At 14 days post‐inoculation the transgenic plant line reached the maximum virus accumulation measured by ELISA. Those plants also showed a 1 week delay of virus accumulation by comparison with control plants. After reaching the peak at 14 days post‐inoculation the virus titre in line 232 decreased with respect to control plants, until the end of the experiment. These results are compatible with the presence of an RNA‐mediated resistance mechanism.