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Genomic Diversity of Field Isolates of Burkholderia solanacearum in Japan
Author(s) -
Ito S.,
Fujji T.,
Uushijima Y.,
Tanaka S.,
KameyaIwaki M.,
Yoshiwara S.,
Kishi F.
Publication year - 1996
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1996.tb00332.x
Subject(s) - biology , rapd , burkholderia , genomic dna , genetic diversity , genetics , polymerase chain reaction , genome , restriction fragment length polymorphism , dna , microbiology and biotechnology , gene , bacteria , population , demography , sociology
Genomic differences between 18 isolates of Burkholderia solanacearum originated from diseased plants cultivated in nine different agricultural fields were examined by using four DNA‐based methods: repetitive DNA polymorphism analysis, restriction‐site analysis of polymerase chain reaction products, random amplified polymorphic DNA (RAPD) analysis, and macrorestriction analysis. Genomic diversity among the isolates was detected by RAPD and macrorestriction analysis. The latter revealed the regional variation of the genome structure among the isolates, suggesting that B. solanacearum populations consist of a number of independent clonal lines.